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| The effect of demineralized bone matrix implants loaded with syngenic mesenchymal stem cells on the healing of cranial segmental bone defects in rats. |
| Topic:
Mesenchymal stem cells (biology and clinical use) |
P. Krouglyakov, I. Sokolova, N. Zinkova, S. Vijde, T. Kreslavsky, N. Cherednichenko, T. Kislyakova, D. Polyntsev;
Trans-Technology, Ltd, Saint-Petersburg, RUSSIAN FEDERATION. |
| Presentation Number: 177 |
| Poster Board Number: 177 |
| Keywords:
mesenchymal stem cells, demineralized bone matrix, cranial defects |
The use of bone substitutes to help repair of damaged bone tissues is of particular clinical interest. One aspect of tissue engineering is concerned with the formation of three-dimensional tissue substitutes by culturing cells on natural or synthetic polymer scaffolds. To help repair of critical size cranial bone defects we attempted MSC-based cell therapy approach.
Experiments were carried out on inbred Wistar-Kyoto rats. MSCs were isolated, expanded in culture and phenotyped by flow cytometry analysis of surface staining for negative (CD 34 and CD 45) and positive (CD 90) markers. Demineralized bone matrixes (DMBs) from parietal rat and chicken bones were used as bone substitutes. Parts of DMBs was loaded with the singenic MSCs. Matrixes with or without MSCs or metal plates were implanted in critical size cranial bone defects in rats.
Histological examination showed obvious de novo bone formation and angiogenic activity in the place of osseous defect when MSC-loaded matrixes were implanted. MSC-loaded rat DBM implantation resulted in lamellar bone formation. This bone was at least partly united with recipient bone. MSC suppressed post-implantation inflammation in the case of MSC-loaded chicken DBMs.
Singenic MSC that had been loaded onto DBMs elicited the healing of critical-sized bone defects in rat. |
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